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Ekstracelulārā matriksa sintēzes un degradācijas loma ādas dzīšanā un tās analīze biotehnoloģiski veidotos ādas audos

2015

Darba mērķis ir izstrādāt ādas audu modeli in vitro, balstītu uz ādas šūnu kokultūru kultivēšanu trīsdimensionālā fibrīna matriksā, kā arī noteikt izveidotajos audos ekstracelulārā matriksa (ECM) komponentu sintēzi un degradāciju. Papildus tika ievākti arī hronisku brūču eksudātu paraugi, kuriem tika veikta dažādu – ar brūču dzīšanu saistītu marķieru analīze. Mērķa sasniegšanai tika izmantotas šūnu, molekulārās bioloģijas un imunoloģijas metodes. Darbā iegūtie rezultāti parāda ECM komponentu, to degradējošo enzīmu un augšanas faktoru produkcijas izmaiņas audu kultivēšanas laikā un ļauj izvēlēties optimālu ādas audu modeļsistēmas veidošanas un kultivēšanas metodiku. Darbs tika izstrādāts Pau…

ekstracelulārais matriksseksudātibrūču dzīšanaBioloģijakokultūras3D ādas audu modelis

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PEPhysical Sciences and Engineering
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LSLife Sciences
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