6533b835fe1ef96bd129f451

RESEARCH PRODUCT

Liquid chromatographic determination of toxigenic secondary metabolites produced by Fusarium strains.

R. MateoMisericordia JiménezM.j. HinojoJ.j. MateoA. Llorens

subject

FusariumTrichotheceneBiochemistrySensitivity and SpecificityAnalytical Chemistrychemistry.chemical_compoundFusariumFumonisinSolid phase extractionMycotoxinDerivatizationZearalenoneChromatography High Pressure LiquidChromatographybiologyOrganic Chemistryfood and beveragesGeneral MedicineReversed-phase chromatographyMycotoxinsbiology.organism_classificationChromatography Ion ExchangeSpectrometry FluorescencechemistryCalibrationSpectrophotometry UltravioletEdible Grain

description

Various liquid chromatographic methods used in the analysis of mycotoxins (zearalenone, trichothecenes and fumonisins) produced by Fusarium species were compared in this work. The results demonstrate the suitability of modern clean-up procedures employing multifunctional MycoSep and immunoaffinity columns although these methods are more expensive than conventional methodologies for clean-up. HPLC with both fluorescence and photodiode array detection is a suitable technique for the analysis of toxic secondary metabolites produced by Fusarium species; different derivatisation strategies have been studied to improve the sensitivity of the technique because of the low concentration of these metabolites in contaminated food. The utility of the proposed methodology was assessed in cereal cultures of various Fusarium strains.

yearjournalcountryeditionlanguage
2002-05-01Journal of chromatography. A
10.1016/s0021-9673(02)00214-5https://pubmed.ncbi.nlm.nih.gov/12075928