6533b835fe1ef96bd129f485

RESEARCH PRODUCT

Resonant X-ray scattering in biological structure research

Heinrich B. Stuhrmann

subject

Quantitative Biology::BiomoleculesNuclear magnetic resonanceAbsorption edgeAnomalous scatteringScatteringChemistryDynamic nuclear polarisationNeutron scatteringAbsorption (electromagnetic radiation)Molecular physicsLight scatteringDiffractometer

description

The use of anomalous X-ray scattering of light elements like sulfur and phosphorus is of particular interest in biological structure research. These elements serve as native labels in proteins, nucleic acids and membranes. Their medium scattering power is drastically changed at their K absorption edges at wavelengths between 5 and 6 A where X-ray absorption excludes the use of open air diffractometers. The construction of a new diffractometer tunable to wavelengths between 1.2 and 7 A is presented. First results of anomalous scattering from sulfur in bacteriorhodopsin near the K absorption edge have been obtained recently. Their possible impact on crystallography will be considered. A comparison with nuclear spin dependent neutron scattering is given.

yearjournalcountryeditionlanguage
2007-11-04
https://doi.org/10.1007/bfb0111239