6533b835fe1ef96bd129f536
RESEARCH PRODUCT
In vitro antitumor effects of the cold-water extracts of Mediterranean species of genus Pleurotus (higher Basidiomycetes) on human colon cancer cells
Odessa SchillaciGiuseppe VenturellaAlessandro SaittaGiacomo De LeoA. FlugyRiccardo AlessandroMaria Letizia GarganoSimona FontanaAlessandra Cannizzarosubject
medicinal mushrooms Pleurotus eryngii var. ferulae Pleurotus nebrodensis human colon cancer antitumor activityCell SurvivalApoptosisPleurotusApplied Microbiology and BiotechnologySettore BIO/13 - Biologia ApplicataCell Line TumorVegetablesDrug DiscoveryExtracellularHumansPleurotus eryngiiCell Proliferationbcl-2-Associated X ProteinPharmacologyPleurotus nebrodensisPleurotusbiologyPlant ExtractsKinasebiology.organism_classificationAntineoplastic Agents PhytogenicIn vitroProto-Oncogene Proteins c-bcl-2BiochemistryApoptosisColonic NeoplasmsPhosphorylationdescription
The aim of this study was to evaluate whether the cold-water extracts of Pleurotus eryngii var. ferulae (CWE-Pef) and Pleurotus nebrodensis (CWE-Pn), 2 of the most prized wild and cultivated edible mushrooms, can affect the tumor phenotype of human colon cancer HCT116 cells. Our results showed that treatment with CWE- Pef and CWE-Pn resulted in a significant inhibition of the viability of HCT116 cells and promoted apoptosis, as also demonstrated by the increase of Bax-to-Bcl-2 messenger RNA ratio. Moreover, we observed that both extracts were able to inhibit cell migration and to affect homotypic and heterotypic cell-cell adhesion. It also was found that treatment with CWE-Pef and CWE-Pn negatively modulated the phosphorylation of the protein tyrosine as well as the phosphorylation levels of extracellular signal-regulated kinase 1/2. In conclusion, the in vitro antitumor effects of CWE-Pef and CWE-Pn indicate that they can be considered as possible sources for new alternative therapeutic agents for cancer treatment.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 |