6533b835fe1ef96bd129f5b1

RESEARCH PRODUCT

Comparison of several methods used for the determination of cephalosporins. Analysis of cephalexin in pharmaceutical samples

Adela Sevillano CabezaLuisa Gallo MartinezPilar Campíns Falcó

subject

Clinical BiochemistryPharmaceutical ScienceCapsulesHigh-performance liquid chromatographyAnalytical ChemistryAbsorbancechemistry.chemical_compoundIodometrySpectrophotometryDrug Discoverypolycyclic compoundsmedicineChromatography High Pressure LiquidSpectroscopyAntibacterial agentSecond derivativeCephalexinChromatographymedicine.diagnostic_testChemistrydigestive oral and skin physiologyTitrimetryNinhydrinCephalosporinsSolutionsStandard additionNinhydrinIndicators and ReagentsSpectrophotometry Ultraviolet

description

The precision of UV absorbance of intact and acid degraded cephalosporins, ninhydrin, high performance liquid chromatography and iodometric methods used for analysis of cefoxitin, cefotaxime, cephazolin and cephalexin were compared. To obtain the calibration graphs the analytical signal used were: absorbance, first derivative absorbance, second derivative absorbance and H-point Standard Additions Method by using absorbance values at two selected wavelengths as analytical signal. These methods and calibration graphs were also used for the determination of cephalexin in pharmaceutical samples.

yearjournalcountryeditionlanguage
2002-06-14Journal of Pharmaceutical and Biomedical Analysis
https://doi.org/10.1016/s0731-7085(02)00089-4