Effects of manganese injected into rat nostrils: implications for in vivo functional study of olfaction using MEMRI.

6533b835fe1ef96bd129f664

RESEARCH PRODUCT

Effects of manganese injected into rat nostrils: implications for in vivo functional study of olfaction using MEMRI.

Gérard Coureaud Benoist Lehallier Yves Maurin Jean-marie Bonny

subject

Male Pathology Brain activity and meditation [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition Contrast Media Stimulation Pharmacology MESH : Behavior Animal MEMRI Manganese 030218 nuclear medicine & medical imaging MESH: Magnetic Resonance Imaging 0302 clinical medicine MESH: Smell MESH: Behavior Animal MESH: Animals MESH: Administration Intranasal MESH : Olfactory Bulb medicine.diagnostic_test Behavior Animal Chemistry MESH : Rats Magnetic Resonance Imaging Olfactory Bulb Smell Dose Toxicity MESH: Image Enhancement MESH: Olfactory Bulb medicine.medical_specialty MESH: Rats MESH : Male Biomedical Engineering Biophysics MESH: Manganese Olfaction MESH : Rats Wistar 03 medical and health sciences Primary olfactory cortex In vivo MESH : Magnetic Resonance Imaging MESH: Contrast Media medicine Animals Radiology Nuclear Medicine and imaging Rats Wistar Administration Intranasal MESH : Contrast Media Behavior Manganese Toxicity MESH : Administration Intranasal Magnetic resonance imaging MESH: Rats Wistar Image Enhancement Olfaction MESH: Male Rats Odor Rat MESH : Smell MESH : Image Enhancement MESH : Animals MESH : Manganese [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition 030217 neurology & neurosurgery

description

WOS: 000298212500007; International audience; Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for visualizing neuronal pathways and mapping brain activity modulation. A potential drawback of MEMRI lies in the toxic effects of manganese (Mn), which also depend on its administration route. The aim of this study was to analyze the effects of Mn doses injected into the nostrils of rats on both olfactory perception and MRI contrast enhancement. For this purpose, doses in the range 0-8 μmol MnCl(2) were tested. Behavioral items were quantified with and without odor stimulation during the first 2 h following Mn injection. The MRI study was performed after 16 h of intermittent olfactory stimulations. Behavioral results showed that, during the early period following Mn administration, spontaneous motor activity was not affected, while odor-related behaviors were dose-dependently reduced. MRI results showed that, in the primary olfactory cortex, contrast was rapidly enhanced for Mn doses up to 0.3 μmol and very slowly above. This dose of 0.3 μmol Mn can thus be taken as the optimal dose for injection into rat nostrils to ensure a reproducible contrast in MRI studies while sparing olfactory perception.

year	journal	country	edition	language
2011-04-18

10.1016/j.mri.2011.08.009 https://hal-univ-bourgogne.archives-ouvertes.fr/hal-00723250