6533b835fe1ef96bd129f6e5

RESEARCH PRODUCT

A1 receptors mediate adenosine inhibitory effects in mouse ileum via activation of potassium channels.

subject

description

Abstract Aims We investigated the effects induced by exogenous adenosine on the spontaneous contractile activity of the longitudinal muscle of a mouse ileum, the receptor subtypes activated, the involvement of enteric nerves and whether opening of K + channels was a downstream event leading to the observed effects. Main methods Mechanical responses of the mouse ileal longitudinal muscle to adenosine were examined in vitro as changes in isometric tension. Key findings Adenosine caused a concentration-dependent reduction of the spontaneous contraction amplitude of the ileal longitudinal muscle up to its complete disappearance. This effect induced was markedly reduced by an A 1 receptor antagonist, but not by A 2 and A 3 receptor antagonists and mimicked only by the A 1 receptor agonist. Adenosine uptake inhibitors did not change adenosine potency. A 1 receptor expression was detected at the smooth muscle level. Adenosine responses were insensitive to tetrodotoxin, atropine or nitric oxide synthase inhibitor. Tetraethylammonium and iberiotoxin, BK Ca channel blockers, significantly reduced adenosine effects, whilst 4-aminopyridine, a K v blocker, apamin, a small conductance Ca 2+ -activated K + (SK Ca ) channel blocker, charybdotoxin, an intermediate conductance Ca 2+ -activated K + (IK Ca ) and BK Ca channel blocker, or glibenclamide, an ATP-sensitive K + channel blocker, had no effects. The combination of apamin plus iberiotoxin caused a reduction of the purinergic effects greater than iberiotoxin alone. Significance Adenosine acts as an inhibitory modulator of the contractility of mouse ileal longitudinal muscle through postjunctional A 1 receptors, which in turn would induce opening of BK Ca and SK Ca potassium channels. This study would provide new insight in the pharmacology of purinergic receptors involved in the modulation of the gastrointestinal contractility.