Nanodaļiņu nogādāšana uz audzēju šūnām mezenhimālo cilmes šūnu un MDA-MB-231 šūnu 3D kopkultūras modelī

6533b835fe1ef96bd129f8f9

RESEARCH PRODUCT

Nanodaļiņu nogādāšana uz audzēju šūnām mezenhimālo cilmes šūnu un MDA-MB-231 šūnu 3D kopkultūras modelī

Sabīne Plūduma

subject

CD90 MDA-MB-231 nanodaļiņas Farmācija QD655 mezenhimālās cilmes šūnas

description

Nanodaļiņas tiek uzskatītas par potenciālām transporta molekulām, kuras varētu nogādāt pretvēža zāles tieši uz audzēju šūnām. Bakalaura darba mērķis bija analizēt vai notiek nanodaļiņu pārnese uz audzēja šūnām mezinhimālo cilmes šūnu un MDA-MB-231 šūnu 3D kopkultūru modelī. Abas šūnu kultūras tika kultivēts S10 barotnē. Šūnu kultūru diferenciēšanai tika izvēlēts CD90 marķieris. MCŠ tika iezīmētas ar QD655 un pēc 6h inkubācijas tika veikta plūsmas citometrijas analīze. 3D kopkultūras modelis tika izveidots izmantojot PolyHEMA pārklājumu. QD pārnese uz vēža šūnām tika analizēta ar plūsmas citometriju pēc 48h kultivēšanas 3D kopkultūras modelī. 97% MCŠ 2D kultūrā pēc 6h inkubācijas uzņem QD655, taču 3D kultūrā tikai 7% šūnu saglabā QD iezīmi. 3D kopkultūras modelī apmēram 5% vēža šūnu uzņem QD655.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/32828

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