6533b835fe1ef96bd129fd80

RESEARCH PRODUCT

Small angle neutron scattering study of the quaternary structure of haemocyanin of Rapana thomasiana

Fabio TrioloRichard H. HeenanVito Graziano

subject

Inorganic ChemistryCrystallographyRapana thomasianaSeries (mathematics)ChemistryOrganic ChemistryAnalytical chemistryMoleculeProtein quaternary structureSmall-angle neutron scatteringProtein concentrationSpectroscopyAnalytical Chemistry

description

Abstract Small angle neutron scattering (SANS) experiments were performed on a series of solutions of haemocyanin (Hc) of Rapana thomasiana . In a first series of solutions, by changing the external contrast a model of the molecule that is apparently in disagreement with electron microscopy (EM) measurements was obtained. The apparent disagreement was resolved by performing experiments on a series of variable protein concentration solutions in D 2 O. The physical parameters of the model, extrapolated to zero concentration, are in excellent agreement with the EM results obtained on samples more dilute than those examined with SANS.

yearjournalcountryeditionlanguage
1996-09-01Journal of Molecular Structure
https://doi.org/10.1016/s0022-2860(96)09294-0