6533b835fe1ef96bd129feae
RESEARCH PRODUCT
The Xenopus Oocyte as an Ectopic Expression System for the Selection of Protein Isoform-Specific Antibodies
Sabine SewingMartin StockerSabine KurzOlaf PongsSigrid Reinhardt-maelickesubject
Gene isoformProtein isoformPotassium ChannelsProtein familymedicine.drug_classRecombinant Fusion ProteinsXenopusNerve Tissue ProteinsBiologyMonoclonal antibodyEpitopeMiceXenopus laevisAntigenAntibody SpecificitymedicineAnimalsPharmacologyMice Inbred BALB CHybridomasAntibodies Monoclonalbiology.organism_classificationMolecular biologyOocytesFemaleEctopic expressiondescription
A panel of Xenopus oocytes, each injected with cRNA coding for one specific isoform of the rat brain RCK family of voltage gated potassium channel proteins, was employed to screen for isoform-specific monoclonal antibodies. Several days after injection, cryosections of embedded oocytes were produced and were employed in immunohistochemical analysis of antibody binding. Of the advantageous properties of the assay, it employs the native antigen, it can be applied to homooligomeric and heterooligomeric proteins, and cryosections of the same batch can be stored frozen for later tests. The method may be advantageous also for the selection of isoform-specific antibodies of other protein families.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-01-01 | Journal of Receptor Research |