6533b835fe1ef96bd129ff4b

RESEARCH PRODUCT

ASTROCYTES SHED EXTRACELLULAR VESICLES THAT CONTAIN FIBROBLAST GROWTH FACTOR-2 AND VASCULAR ENDOTHELIAL GROWTH FACTOR.

Gabriella SchieraAntonia Maria Rita IngrassiaMarco MineoItalia Di LiegroPatrizia ProiaGiovanni SavettieriGraziella Santoro

subject

Vascular Endothelial Growth Factor ACellFluorescent Antibody TechniqueBiologyFibroblast growth factorCulture Media Serum-Freechemistry.chemical_compoundWestern blotSettore BIO/10 - BiochimicaGlial Fibrillary Acidic ProteinGeneticsmedicineAnimalsSecretionFibroblastCells Culturedmedicine.diagnostic_testVesicleIntegrin beta1Secretory VesiclesGeneral MedicineCell biologyRatsVascular endothelial growth factorastrocytesextracellular vesicle sheddingfibroblastic growth factors-2Protein Transportmedicine.anatomical_structureMembrane proteinchemistryAstrocytesFibroblast Growth Factor 2

description

An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secondary to myelin damage, as generally assumed. SPMS serum affects the permeability of the BBB model, as indicated by the decrease of the transendothelial electrical resistance (TEER). Moreover, as shown by both immunofluorescence and Western blot analyses, BBB breaking is accompanied by a decrease of the synthesis as well as the peripheral localization of occludin, a structural protein of the tight junctions that are responsible for BBB properties.

yearjournalcountryeditionlanguage
2007-12-22
http://hdl.handle.net/10447/36891