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RESEARCH PRODUCT

Additional file 1: of Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

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A) PCR-array results: Mean and SEM of the fold changes of the 45 selected genes in Sparicotyle infected fish (n = 10) calculated relative to the control uninfected group (n = 10) in parasitized gill portions (with parasite presence), non-parasitized gill portions (no parasite present) and spleen. Student’s t test or Mann-Whitney-Wilcoxon test when normality conditions were not met were used to calculate p values comparing expression levels of control and infected animals per gene. Dark red and green indicate significantly up- or down-regulated genes, respectively, with p