6533b835fe1ef96bd12a0078

RESEARCH PRODUCT

Identification of a genetic contamination in a commercial mouse strain using two panels of polymorphic markers

Heidi HahnF NitzkiA KrugerK ReifenbergLeszek Wojnowski

subject

Genetic MarkersGenotypeMice Inbred StrainsBiologyQuantitative trait locusMice03 medical and health sciences0302 clinical medicineInbred strainGenotypeAnimalsGenetic TestingAlleleSimple sequence length polymorphism030304 developmental biologyGeneticsMice Inbred BALB C0303 health sciencesPolymorphism GeneticBase SequenceGeneral VeterinaryGenetic Carrier ScreeningStrain (biology)Mice Inbred C57BLGenetic marker030220 oncology & carcinogenesisAnimal Science and ZoologyGenetic monitoring

description

Rapid detection of genetic contamination is critical in mouse studies involving inbred strains. During a Quantitative Trait Locus (QTL) study using simple sequence length polymorphism (SSLP) markers, we noticed heterozygosity at some loci of a commercially available inbred C57BL/6N mouse strain, suggesting a contamination by another mouse strain. A panel of 100 single-nucleotide polymorphism (SNP) markers was used to confirm and specify the genetic contamination suspected. Retrospective analyses demonstrated that the contamination took place as early as autumn 2003 and has persisted ever since at a fairly constant level. Contaminating alleles most probably originated from a DBA strain. Our data demonstrate the suitability of SNP markers for rapid detection and identification of the source of genetic contamination. Further, our results show the importance of a state-of-theart genetic monitoring of the authenticity of murine inbred strains.

yearjournalcountryeditionlanguage
2007-04-01Laboratory Animals
https://doi.org/10.1258/002367707780378104