6533b835fe1ef96bd12a0127

RESEARCH PRODUCT

A simple method to estimate the isoelectric point of modified Tomato bushy stunt virus (TBSV) particles

Wolfgang GebauerGabriela KrczalMario BraunChristiane ZieglerKajohn BoonrodChristine Müller-renno

subject

0301 basic medicineTombusvirusSurface PropertiesvirusesClinical BiochemistryBuffersBiologyBiochemistryVirusAnalytical ChemistryDiffusionTombusvirus03 medical and health sciencesIsoelectric PointElectrophoresis Agar GelIsoelectric focusingVirionfood and beveragesHydrogen-Ion Concentrationbiology.organism_classificationMolecular biologyElectrophoresis030104 developmental biologyIsoelectric pointCapsidBiophysicsParticleCapsid ProteinsPeptidesTomato bushy stunt virus

description

We present a simple method to estimate the isoelectric point (pI) of Tomato Bushy Stunt particles. We demonstrate that the combination of agarose gels with different pH buffers can be used to electrophorese the virus particles and their migration patterns can be compared. This method allows us to estimate the pI of the virus particles (wild type, wt, and genetically modified particles) and to monitor the effect of the pI of modified peptide side chains of the viral capsid subunit on the pI of the whole virus particle.

yearjournalcountryeditionlanguage
2017-05-23ELECTROPHORESIS
https://doi.org/10.1002/elps.201700209