6533b836fe1ef96bd12a0bd7
RESEARCH PRODUCT
Age-related changes in antioxidant defence mechanisms and peroxidation in isolated hepatocytes from spontaneously hypertensive and normotensive rats
Evelyne VéricelMichel NarceMichel LagardeJean-pierre PoissonLionel Ulmannsubject
MaleAgingAntioxidantmedicine.medical_treatmentClinical BiochemistryDefence mechanisms030204 cardiovascular system & hematologyRats Inbred WKYAntioxidantsLipid peroxidationchemistry.chemical_compound0302 clinical medicineMalondialdehydeRats Inbred SHRVitamin E[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM]chemistry.chemical_classification0303 health sciencesbiologyGlutathione peroxidaseGeneral MedicineThiobarbituratesGlutathione[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM]GSH-PxLiverHypertensionhepatocytesmedicine.medical_specialtyClinical chemistry[SDV.CAN]Life Sciences [q-bio]/Cancer03 medical and health sciences[SDV.CAN] Life Sciences [q-bio]/CancerInternal medicinemedicineAnimalsMolecular Biology030304 developmental biologyGlutathione PeroxidaseVitamin ECell BiologyGlutathioneEnzyme assayRats[SDV.AEN] Life Sciences [q-bio]/Food and NutritionEndocrinologychemistryagebiology.proteinLipid Peroxidation[SDV.AEN]Life Sciences [q-bio]/Food and Nutritiondescription
International audience; The effects of age and hypertension on the antioxidant defence systems and the lipid peroxidation in rat isolated hepatocytes were studied. Four different age groups (1, 3, 6 and 12 months) were considered in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Age-associated changes were observed on vitamin E status, glutathione (GSH) level, MDA formation and glutathione peroxidase (GSH-Px) activity in both strains. Maximal levels or activities of these parameters were found at 3 and 6 months, except for MDA which was low at 3 months. Then, a fall was observed at 12-month-old compared to 6-month values. In addition, GSH-Px activity was significantly lower in SHR than in WKY rats, except at the age of one month. The decrease of this enzyme activity could induce an increased cellular generation of radical species and lipid peroxidation, which might be link to hypertension.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-03-16 |