6533b836fe1ef96bd12a1238

RESEARCH PRODUCT

Development of microsatellite markers for the critically endangered Limonium dufourii (Girard) Kuntze (Plumbaginaceae)

Fernando González-candelasM. Palop-esteban

subject

Geneticseducation.field_of_studyEcologybiologyLimoniumPopulationLocus (genetics)biology.organism_classificationBiochemistryGeneral Biochemistry Genetics and Molecular BiologyCritically endangeredEvolutionary biologyApomixisGenetic structureMicrosatelliteAlleleeducation

description

Limonium dufourii is an endemic plant from the eastern Mediterranean coast of Spain with a triploid chromosome number and apomictic reproduction. We have isolated and characterized 13 polymorphic microsatellite loci from an enriched library in order to investigate its population genetic structure. Simple sequence repeat (SSR) loci were screened in 120 individuals from the six extant populations of this species. They show an average of 5.76 alleles per locus, ranging from 2 to 18, with seven loci exhibiting heterozygosities larger than 0.60. Three loci present one single allele in each individual, whereas one locus presents three alleles in every individual analysed.

https://doi.org/10.1046/j.1471-8286.2002.00300.x