6533b836fe1ef96bd12a13aa
RESEARCH PRODUCT
Immune blot analysis of viral surface proteins in serum and liver of patients with chronic hepatitis B virus infection
Georg HessG. GerkenMichael MannsWolfram H. GerlichKarl-hermann Meyer Zum Büschenfeldesubject
AdultMaleHepatitis B virusmedicine.drug_classvirusesImmunoblottingBiologyMonoclonal antibodymedicine.disease_causeVirus03 medical and health sciences0302 clinical medicineViral envelopeVirologymedicineHumansAgedHepatitis Chronic030304 developmental biologyHepatitis B virusHepatitis0303 health sciencesHepatitis B Surface AntigensMiddle AgedHepatitis Bbiology.organism_classificationmedicine.diseaseVirologyMolecular biology3. Good healthBlotBloodInfectious DiseasesLiverHepadnaviridaeCell cultureFemale030211 gastroenterology & hepatologydescription
The small and the middle surface proteins of hepatitis virus form either the virion or the 22 nm particle both of which are secreted. The large surface protein by itself remains cell bound in artificially transfected cell culture unless it is accompanied by an excess of the smaller protens. Its behavior in vivo is not yet well studied. Using specific monoclonal antibodies for immunoblotting, we found an abundance of small surface protein in the serum of chronic virus carriers and moderate amounts in the liver irrespective of viremia. The large surface protein was present in the serum and the liver of viremic carriers. In nonviremic carriers, the large protein was absent from serum, but in the liver a shorter form of the large protein was readily detectable. These findings suggest a complex regulatory mechanism of the viral surface protein depending on the expression of other viral gene products.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-12-01 | Journal of Medical Virology |