6533b836fe1ef96bd12a1485
RESEARCH PRODUCT
Cellular expression of α4 subunit mRNA of the nicotinic acetylcholine receptor in the developing rat telencephalon
Alfred MaelickeHannsjörg SchröderJ. GrunwaldC.-h. OstermannAndrea WeversSigrid ReinhardtDietrich E. Lorkesubject
MaleTelencephalonmedicine.medical_specialtyGene ExpressionIn situ hybridizationReceptors NicotinicBiologyHippocampusInternal medicinemedicineAnimalsRNA MessengerRats WistarCholinergic neuronIn Situ HybridizationAcetylcholine receptorCerebral CortexNeocortexCerebrumGeneral NeuroscienceDentate gyrusRatsNicotinic acetylcholine receptorEndocrinologymedicine.anatomical_structurenervous systemCholinergicFemalesense organsdescription
By contrast to adult brain, little is known on the development of nicotinic acetylcholine receptor (nAChR) expression. Using a digoxigenin-labeled riboprobe for in situ hybridization, alpha 4 nAChR subunit mRNA expression was studied in embryonic and postnatal rat neocortex and hippocampus where it was transiently increased in neuronal subpopulations and preceded cholinergic fiber ingrowth. alpha 4 expression was increased in neocortical layer VIb between E20 and P2 and, about birth, in dentate gyrus granule cells subsequently decreasing to adult levels. nAChR mRNA expression is increased at the developing neuromuscular endplate preceding cholinergic innervation which triggers changes in non-alpha nAChR isoform expression. It has to be elucidated whether similar changes may occur in the telencephalon.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-06-02 | Neuroscience Letters |