6533b836fe1ef96bd12a14da

RESEARCH PRODUCT

Candida albicans biofilms on different materials for manufacturing implant abutments and prostheses

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[EN] Background: Morphological, physical and chemical properties of both implants and prostheses can determine the biofilm formation on their surface and increase the risk of biological complications. The aim of this study was to evaluate the capacity of biofilm formation of Candida albicans on different materials used to manufacture abutments and prostheses. Material and Methods: Biofilm formation was analyzed on cp grade II titanium, cobalt-chromium alloy and zirconia, silicone, acrylic resin (polymethylmethacrylate) and nano-hybrid composite. Some samples were partially covered with lithium disilicate glass ceramic to study specifically the junction areas. C. albicans was incubated in a biofilm reactor at 37 °C with agitation. The biofilm formation was evaluated at 24 and 48 hours. In addition, the morphology of the biofilm was evaluated by scanning electron microscopy. Results: C. albicans developed biofilms on the surface of all materials tested. Cobalt-chromium alloy showed the lowest density of adhered biofilm, followed by zirconia and titanium. Silicone and resin showed up to 20 times higher density of biofilm. A higher biofilm formation was observed when junctions of materials presented micropores or imperfections. Conclusions: The biofilm formed in the three materials used in the manufacture of abutments and prostheses showed no major differences, being far less dense than in the resins. Two clinical recommendations can be made: to avoid the presence of resins in the subgingival area of implant prostheses and to design prostheses placing cobalt-chromium alloy/ceramic or titanium/ceramic junctions as far as possible from implants. ES, GQ, and EE have received research grant support from the Consejería de Educación, Universidades e Investigación del Gobierno Vasco-Eusko Jaurlaritza (GIC15 78 IT-990-16), the Ministerio de Economía y Competitividad del Gobierno de España, MINECO (SAF2017-86188-P) and the Universidad del País Vasco/Euskal Herriko Unibertsitatea, UPV/EHU (UFI 11/25). The authors report no conflicts of interest related to this study. The authors thank for technical and human support provided by Ramón Santamaría (Corus dental) and Analytical and High-Resolution Microscopy Service in Biomedicine of SGIker-UPV/EHU.