6533b836fe1ef96bd12a15b0
RESEARCH PRODUCT
Localization of mitochondrial Hsp56 chaperonin during sea urchin development.
Fabrizio GianguzzaM. PattiMaria Carmela RoccheriElena CarraAnna Maria RinaldiMaria Agnellosubject
Nuclear geneEmbryo NonmammalianBlotting WesternBiophysicsMitochondrionCell FractionationBiochemistryParacentrotus lividusChaperoninTacrolimus Binding Proteinsbiology.animalAnimalsMolecular BiologySea urchinbiologyCell Biologybiology.organism_classificationMolecular biologyImmunohistochemistryCell biologyMitochondriaMitochondrial matrixCytoplasmSea UrchinsHSP60Molecular Chaperonesdescription
We have previously demonstrated that Paracentrotus lividus nuclear genome encodes for the heat shock inducible chaperonin homolog Hsp 56 (1) and that the mature protein is localized in the mitochondrial matrix (2). In this paper we report that constitutive Hsp56 is maternally inherited, in fact it is present in the in unfertilized eggs, and that it has a perinuclear specific localization during cleavage. In the later stages both the constitutive and the heat shock inducible chaperonin has a specific territorial distribution. Moreover following heat shock, the Hsp56 appears in the cytoplasm and in the postmitochondrial supernatant beside the mitochondrial fraction.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-10-01 | Biochemical and biophysical research communications |