6533b836fe1ef96bd12a1628
RESEARCH PRODUCT
Comparison of hemolytic activity of the intermediate subunit of Entamoeba histolytica and Entamoeba dispar lectins.
Hiroshi TachibanaKentaro KatoTakashi MakiuchiXun-jia ChengXun-jia Chengsubject
0301 basic medicineErythrocytesTime Factorslcsh:MedicineProtein Sequencingmedicine.disease_causePathology and Laboratory MedicineBiochemistrylaw.inventionEntamoebafluids and secretionslawLectinsMedicine and Health SciencesRecombinant Protein Purificationlcsh:ScienceProtozoansMultidisciplinarybiologyPseudomonas AeruginosaRecombinant ProteinsBacterial PathogensMedical MicrobiologyRecombinant DNAPathogensResearch ArticleProtein PurificationProtein subunitDisparResearch and Analysis MethodsReal-Time Polymerase Chain ReactionMicrobiologyHemolysisMicrobiologyEntamoeba Histolytica03 medical and health sciencesEntamoeba histolyticaPseudomonasParasite Groupsparasitic diseasesmedicineAnimalsTrophozoitesHemoglobinGene SilencingHorsesMolecular Biology TechniquesSequencing TechniquesEscherichia coliMolecular BiologyMicrobial PathogensBacterialcsh:REntamoebaOrganismsLectinBiology and Life SciencesProteinsbiology.organism_classificationParasitic Protozoansdigestive system diseasesProtein Subunits030104 developmental biologybiology.proteinProtozoaParasitologylcsh:QApicomplexaPurification Techniquesdescription
Galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica has roles in pathogenicity and induction of protective immunity in rodent models of amoebiasis. Recently, the intermediate subunit of the lectin, Igl1, of E. histolytica has been shown to have hemolytic activity. However, the corresponding lectin is also expressed in a non-virulent species, Entamoeba dispar, and another subunit, Igl2, is expressed in the protozoa. Therefore, in this study, we compared the activities of Igl1 and Igl2 subunits from E. histolytica and E. dispar using various regions of recombinant Igl proteins expressed in Escherichia coli. The recombinant E. dispar Igl proteins had comparable hemolytic activities with those of E. histolytica Igl proteins. Furthermore, Igl1 gene-silenced E. histolytica trophozoites showed less hemolytic activity compared with vector-transfected trophozoites, indicating that the expression level of Igl1 protein influences the activity. These results suggest that the lower hemolytic activity in E. dispar compared with E. histolytica reflects the lower expression level of Igl1 in the E. dispar parasite.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-07-27 | PLoS ONE |