6533b837fe1ef96bd12a1d84

RESEARCH PRODUCT

Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes.

Edward A. LemkeGemma Estrada GironaChristopher D. Reinkemeier

subject

Computer scienceComputational biology010402 general chemistryProtein Engineering01 natural sciencesGenomeArticle03 medical and health sciencesSynthetic biologyRNA TransferOrganelleChlorocebus aethiopsAnimalsHumansRNA MessengerCaenorhabditis elegansCodon030304 developmental biologyOrganelles0303 health sciencesMultidisciplinaryLysineHEK 293 cellsCell MembraneRNAProtein engineeringGenetic code0104 chemical sciencesHEK293 CellsGenetic CodeProtein BiosynthesisCOS CellsMethanosarcinaSynthetic BiologyRibosomesArtificial Organelles

description

How to make an organelle in eukaryotes A key step in the evolution of complex organisms like eukaryotes was the organization of specific tasks into organelles. Reinkemeier et al. designed an artificial, membraneless organelle into mammalian cells to perform orthogonal translation. In response to a specific codon in a selected messenger RNA, ribosomes confined to this organelle were able to introduce chemical functionalities site-specifically, expanding the canonical set of amino acids. This approach opens possibilities in synthetic cell engineering and biomedical research. Science , this issue p. eaaw2644

yearjournalcountryeditionlanguage
2019-03-29Science (New York, N.Y.)
10.1126/science.aaw2644https://pubmed.ncbi.nlm.nih.gov/30923194