6533b837fe1ef96bd12a1e05

RESEARCH PRODUCT

Partial purification and some properties of a nucleoside phosphotransferase of chick embryos.

Giuseppe CalvarusoGennaro TaibiGiovanni TesoriereRenza Vento

subject

PyrimidineDeoxyribonucleotidesChick EmbryoThymidine KinasePhosphatesSubstrate SpecificityCellular and Molecular Neurosciencechemistry.chemical_compoundNucleoside phosphotransferaseAnimalsNucleotideMolecular BiologyPharmacologychemistry.chemical_classificationPhosphotransferasesNucleosidesCell BiologyRibonucleotidesRibonucleosideChick embryosPhosphateDeoxyribonucleosideEnzymeBiochemistrychemistryMolecular Medicine

description

A nucleoside phosphotransferase purified about 40fold from chick embryos utilizes efficiently as phosphate donors deoxyribonucleoside and pyrimidine ribonucleoside monophosphates, whereas the pyrimidine deoxyribonucleoside appear to be the preferred acceptors of phosphate. The enzyme is very unstable to heat, dilution and dialysis. A marked enhancement in the stability is caused by nucleotides and it seems associated with the formation of an aggregated state of the protein.

yearjournalcountryeditionlanguage
1978-10-15Experientia
10.1007/bf01981409https://pubmed.ncbi.nlm.nih.gov/738391