Tenectin is a novel alphaPS2betaPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila.

6533b837fe1ef96bd12a1f75

RESEARCH PRODUCT

Tenectin is a novel alphaPS2betaPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila.

Anne-laure Bougé Thomas A. Bunch Timmy Kendall Stéphane Fraichard Isabelle Chauvel Hervé Bouhin

subject

Male MESH: Extracellular Matrix Proteins MESH: Drosophila MESH : Immunohistochemistry [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition Integrin Ligands Looping morphogenesis Extracellular matrix chemistry.chemical_compound 0302 clinical medicine MESH: Genitalia Male Morphogenesis MESH: Ligands Drosophila Proteins Wings Animal MESH: Animals Transgenes In Situ Hybridization 0303 health sciences Extracellular Matrix Proteins MESH : Genitalia Male MESH : Ligands Integrin alpha Chains Cell adhesion molecule MESH : In Situ Hybridization Immunohistochemistry 3. Good health Cell biology Larva MESH : Integrin alpha Chains Adhesion Drosophila MESH : Mutation MESH : Transgenes Tenectin Integrin alpha Chains Drosophila Protein Ecdysone Ecdysone MESH: Mutation MESH: Drosophila Proteins MESH : Male Integrin Morphogenesis MESH : Wing MESH: Transgenes Biology Genitalia Male Article 03 medical and health sciences MESH : Extracellular Matrix Proteins MESH: In Situ Hybridization Animals MESH : Drosophila Cell adhesion Molecular Biology 030304 developmental biology MESH : Larva Metamorphosis MESH: Integrin alpha Chains Left–right asymmetry MESH: Immunohistochemistry Cell Biology MESH : Drosophila Proteins MESH: Wing MESH: Male MESH: Morphogenesis chemistry MESH : Morphogenesis Mutation biology.protein MESH : Animals MESH: Larva [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition 030217 neurology & neurosurgery Developmental Biology

description

International audience; Morphogenesis of the adult structures of holometabolous insects is regulated by ecdysteroids and juvenile hormones and involves cell-cell interactions mediated in part by the cell surface integrin receptors and their extracellular matrix (ECM) ligands. These adhesion molecules and their regulation by hormones are not well characterized. We describe the gene structure of a newly described ECM molecule, tenectin, and demonstrate that it is a hormonally regulated ECM protein required for proper morphogenesis of the adult wing and male genitalia. Tenectin's function as a new ligand of the PS2 integrins is demonstrated by both genetic interactions in the fly and by cell spreading and cell adhesion assays in cultured cells. Its interaction with the PS2 integrins is dependent on RGD and RGD-like motifs. Tenectin's function in looping morphogenesis in the development of the male genitalia led to experiments that demonstrate a role for PS integrins in the execution of left-right asymmetry.

year	journal	country	edition	language
2010-04-15

10.1016/j.ydbio.2010.02.008 https://hal-univ-bourgogne.archives-ouvertes.fr/hal-00761472