6533b837fe1ef96bd12a2043
RESEARCH PRODUCT
Neurons and ECM regulate occludin localization in brain endothelial cells
Giovanni SavettieriI. Di LiegroC. CataniaGiulia GiandaliaA. CestelliGiovanna PitarresiLuana LicataGabriella SchieraLibero Italo GiannolaV. De CaroStefania D'agostinosubject
Time FactorsEndothelial cellsCellOccludinTight JunctionsExtracellular matrixRats Sprague-DawleyFetusLamininNeurofilament ProteinsOccludinSettore BIO/10 - BiochimicaGlial Fibrillary Acidic ProteinmedicineAnimalsRNA MessengerCells CulturedBlood-brain barrierNeuronsbiologyTight junctionGeneral NeuroscienceBrainMembrane ProteinsCortical NeuronsExtracellular matrixImmunohistochemistryCell biologyRatsEndothelial stem cellmedicine.anatomical_structureMembrane proteinCell cultureSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoCerebrovascular Circulationbiology.proteinSettore MED/26 - NeurologiaCollagenEndothelium VascularLamininNeurosciencedescription
We report that extracellular matrix and neurons modulate the expression of occludin, one of the main components of tight junctions, by rat brain endothelial cells (RBE4.B). Of the three extracellular matrix proteins which we tested (collagen I, collagen IV, and laminin), collagen IV stimulated at the best the expression of occludin mRNA. The corresponding protein, however, was not synthesized. Significant amounts of occludin accumulated only when RBE4.B cells were cultured on collagen IV-coated inserts, in the presence of cortical neurons, plated on laminin-coated companion wells. Finally, occludin segregated at the cell periphery, only when endothelial cells were co- cultured with neurons for ≥ 1 week. (C) 2000 Lippincott Williams and Wilkins.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-05-03 |