Adventitious shoot regeneration from hypocotyl cultures of service tree(Sorbus domesticaL.)

6533b837fe1ef96bd12a2695

RESEARCH PRODUCT

Adventitious shoot regeneration from hypocotyl cultures of service tree(Sorbus domesticaL.)

subject

chemistry.chemical_classification Sorbus domestica Organogenesis Plant Science Biology biology.organism_classification Hypocotyl Basal shoot Sorbus chemistry Auxin Callus Botany Explant culture

description

Effects of growth regulators and N03:NH4 ratio were investigated to determine the mor- phogenic capacity of hypocotyl explants of service tree (Sorbus domestica L.) Organogenic responses were preceded by an initial callus phase and depended mainly on the auxin type used. NAA induced root differentiation whereas IAA added to media containing BA promoted adventitious shoot regeneration. Best results were obtained with 2.5 μM each of BA and IAA (19% of caulogenic explants). This reponse was increased (up to 44%) by varying nitrate to ammonium ratio from 2:1 to 4:1.

year	journal	country	edition	language
1992-01-01	Journal of Horticultural Science

https://doi.org/10.1080/00221589.1992.11516260

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