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RESEARCH PRODUCT

Successful pregnancy and childbirth after intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient

Antonio PellicerM. MolláA. TejeraJosé RemohíJose Luis De PabloLourdes Muriel

subject

AdultMalemedicine.medical_specialtymedicine.medical_treatmentIntracytoplasmic sperm injectionAndrologyHuman fertilizationPregnancyHumansMedicineSperm Injections IntracytoplasmicGlobozoospermiaAzoospermiaGynecologyAzoospermiaPregnancyIonophoresbusiness.industryFertility Agents MaleObstetrics and GynecologyOocyte activationEmbryomedicine.diseaseCombined Modality TherapyTreatment OutcomeReproductive Medicineembryonic structuresOocytesCalciumFemalebusinessLive BirthEmbryo quality

description

Objective To check the effectiveness of intracytoplasmic sperm injection (ICSI) combined with assisted oocyte activation (AOA) in a globozoospermic patient. Design Case report. Setting Instituto Valenciano de Infertilidad, Valencia, Spain. Patient(s) A patient with globozoospermia. Intervention(s) ICSI was administered in 14 oocytes. ICSI combined with AOA, in which a small amount of calcium was injected followed by calcium ionophore exposure, was done in 9 oocytes. Main Outcome Measure(s) Fertilization rate and embryo quality was assessed in both groups. Result(s) Chemical activation increased fertilization rate (55.6% vs. 35.7%) and the number of embryos with less multinucleation on day 2 (0 vs. 60%). Two embryos generated from AOA were transferred into the uterus (on day 3), resulting in a pregnancy and a healthy newborn. Conclusion(s) The AOA with calcium ionophore treatment improved fertilization rate and quality of the embryos, and was found to be an effective method for AOA in this patient with a low fertilization rate after previous ICSI treatment.

yearjournalcountryeditionlanguage
2007-06-22Fertility and Sterility
https://doi.org/10.1016/j.fertnstert.2007.11.056