6533b837fe1ef96bd12a2871
RESEARCH PRODUCT
Survival and differentiation of embryonic neural explants on different biomaterials
José Luis Gómez RibellesManuel Salmerón SánchezJosé Manuel García-verdugoJuan A. BarciaCristina Martínez RamosAlberto Campillo FernándezM. Monleón PradasJosé Miguel SoriaVeronique Benaventsubject
Materials scienceGanglionic eminenceBiocompatibilityCellular differentiationBiomedical EngineeringBiocompatible MaterialsIn Vitro TechniquesBiomaterialschemistry.chemical_compoundCell MovementMaterials TestingAnimalsNerve TissueProgenitor cellMethyl acrylateStem CellsMetals and AlloysBiomaterialCell DifferentiationEmbryonic stem cellRatsCell biologychemistryCeramics and CompositesEthyl acrylateBiomedical engineeringdescription
Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-06-22 | Journal of Biomedical Materials Research Part A |