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RESEARCH PRODUCT

Spectral transmission of the pig lens: Effect of ultraviolet A+B radiation

subject

description

Summary Objective To determine the spectral transmission curve of the crystalline lens of the pig. To analyse how this curve changes when the crystalline lens is irradiated with ultraviolet A + B radiation similar to that of the sun. To compare these results with literature data from the human crystalline lens. Procedures We used crystalline lenses of the common pig from a slaughterhouse, i.e. genetically similar pigs, fed with the same diet, and slaughtered at six months old. Spectral transmission was measured with a Perkin-Elmer Lambda 35 UV/VIS spectrometer. The lenses were irradiated using an Asahi Spectra Lax-C100 ultraviolet source, which made it possible to select the spectral emission band as well as the intensity and exposure time. Results The pig lens transmits all the visible spectrum (95%) and lets part of the ultraviolet A through (15%). Exposure to acute UV (A + B) irradiation causes a decrease in its transmission as the intensity or exposure time increases: this decrease is considerable in the UV region. Conclusions We were able to determine the mean spectral transmission curve of the pig lens. It appears to be similar to that of the human lens in the visible spectrum, but different in the ultraviolet. Pig lens transmission is reduced by UV (A + B) irradiation and its transmission in the UV region can even disappear as the intensity or exposure time increases. An adequate exposure intensity and time of UV (A + B) radiation always causes an anterior subcapsular cataract (ASC).