6533b837fe1ef96bd12a3156
RESEARCH PRODUCT
MOESM4 of Histone macroH2A1.2 promotes metabolic health and leanness by inhibiting adipogenesis
Valerio PazienzaConcetta PanebiancoFrancesca RappaDomenico MemoliMichela BorghesanSara CannitoAsami OjiGiuseppe MazzaDomenico TamburrinoGiuseppe FusaiRosario BaroneGiulia BolascoFrancesc VillarroyaJoan VillarroyaKiyotaka HatsuzawaFrancesco CappelloRoberta TaralloTomoko NakanishiManlio Vinciguerrasubject
description
Additional file 4. Figure S3. MacroH2A1.1 and macroH2A1.2 expression in 3T3-L1 pre-adipocytes and adipocytes. 3T3-L1 pre-adipocytes with lentiviral-mediates stable expression of GFP, macroH2A1.1-GFP and macroH2A1.2-GFP were induced to differentiate into mature adipocytes as in Fig. 6. At the 1st, 5th and 15th day of differentiation, histones were extracted and processed for immunoblotting with anti-macroH2A1.1, macroH2A1.2 and anti-H3-specific antibodies. Representative blots are shown, together with MW ladder.
year | journal | country | edition | language |
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2016-01-01 |