6533b837fe1ef96bd12a320a
RESEARCH PRODUCT
Comparison of the performance of 2 commercial multiplex PCR platforms for detection of respiratory viruses in upper and lower tract respiratory specimens
Estela GiménezDavid NavarroMario Rodríguez-domínguezJuan Carlos GalánMaría ÁNgeles ClariElisa Costasubject
Microbiology (medical)AdultMaleAcute respiratory tract infectionAdolescentvirusesRespiratory Systemmedicine.disease_causeSensitivity and SpecificityVirusArticleYoung AdultHuman metapneumovirusMultiplex polymerase chain reactionInfluenza A virusmedicineHumansAcute respiratory tract infectionRespiratory Tract InfectionsAgedRetrospective StudiesAged 80 and overRespiratory virusesbiologyHuman bocavirusRespiratory infectionvirus diseasesGeneral MedicineMultiplex PCRMiddle Agedbiology.organism_classificationVirologyrespiratory tract diseasesInfectious DiseasesVirus DiseasesVirusesFemaleNested polymerase chain reactionMultiplex Polymerase Chain Reactiondescription
The performance of the CLART® PneumoVir system with that of the Luminex xTAG RVP Fast v1 assay for detection of most common respiratory viruses in upper and lower tract respiratory specimens (n = 183) from unique patients with influenza-like syndrome or lower tract respiratory infection. Nested PCR coupled to automated sequencing was used for resolution of discrepancies. Fully concordant results were obtained for a total of 122 specimens, whereas 56 specimens gave partially (n = 21) or fully discordant (n = 35) results (Kappa coefficient, 0.62). The overall specificity of the Luminex xTAG RVP Fast v1 assay was slightly higher than that of the CLART® PneumoVir assay for human bocavirus, influenza A virus/H3N2, influenza B virus, human metapneumovirus, and parainfluenza virus, whereas the sensitivity of the latter was higher for most targeted viruses except, notably, for picornaviruses. This was irrespective of either the origin of the respiratory specimen or the age group to which the patients belonged.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-05-01 | Diagnostic Microbiology and Infectious Disease |