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RESEARCH PRODUCT

Comparison of growth & function of endothelial progenitor cells cultured on deproteinized bovine bone modified with covalently bound fibronectin and bound vascular endothelial growth factor

Eik SchiegnitzMartin HellerPeer W. KämmererBilal Al NawasHermann GötzVinay KumarVinay Kumar

subject

Vascular Endothelial Growth Factor ANitric Oxide Synthase Type IIIAngiogenesis0206 medical engineeringNitric Oxide Synthase Type IICell CountEnzyme-Linked Immunosorbent Assay02 engineering and technologyReal-Time Polymerase Chain ReactionCell morphologyAllylamine03 medical and health scienceschemistry.chemical_compound0302 clinical medicineEnosAnimalsHumansMTT assayProgenitor cellCells CulturedCell ProliferationEndothelial Progenitor CellsMicroscopy Confocalbiology030206 dentistrybiology.organism_classification020601 biomedical engineeringMolecular biologyFibronectinsVascular endothelial growth factorFibronectinchemistryBone SubstitutesImmunologybiology.proteinCattleOral Surgery

description

Objectives The objective of this study was to assess and compare the growth and function of Endothelial Progenitor Cells (EPCs) cultured on covalently bonded Vascular Endothelial Growth Factor (VEGF) and covalently bonded Fibronectin (FN) coating on deproteinized bovine bone (DBB) (test samples), compared to non-modified DBB blocks (control sample). Materials and methods The test samples were prepared by plasma polymerization of allylamine onto DBB blocks. Group1 of test samples were prepared with VEGF coating (VEGF-DBB) where as the Group2 test samples were coated with FN (FN-DBB). Non-modified DBB blocks served as a Control. EPCs were isolated and cultivated from buffy coats of peripheral blood of healthy volunteers and cultivated in the different samples and examined at time intervals of 24 h, 3 days, and 7 days. Evaluation of growth by cell count and cell morphology was done using Confocal Laser Scanning Electron Microscopy; vitality and function of cells was assessed using MTT assay and RT-PCR and ELISA for eNOS and iNOS respectively. Results The results of the study show that both VEGF and FN could be successfully immobilized by plasma polymerization onto a complex, porous, three-dimensional structure of DBB. When comparing vital cell coverage, proliferation and function of EPCs, FN-DBB provided more positive values followed by VEGF-DBB as compared to DBB samples. eNOS level were significant higher in VEGF-DBB and FN-DBB when compared to DBB (P = 0.019 and P = 0.002). The difference between VEGF-DBB and FN-DBB was not significant. Conclusions Biomimetic coatings of Fibronectin may clinically relate to faster angiogenesis and earlier healing potential.

yearjournalcountryeditionlanguage
2016-03-19Clinical Oral Implants Research
https://doi.org/10.1111/clr.12832