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RESEARCH PRODUCT

Additional file 5: of Human limbal fibroblast-like stem cells induce immune-tolerance in autoreactive T lymphocytes from female patients with Hashimoto’s thyroiditis

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Immunomodulation mechanism played from BM-MSCs on healthy activated PBMCs. (A) Acitive-caspase-3 detection by FACS revealed weak induction of the apoptotic cascade in activated healthy PBMCs after 72 h of coculture with BM-MSCs. FACS histogram plots are representative of five experiments of identical design. (B) The expression percentage of the three activation markers (CD28, PD-1, CD69) in activated healthy PBMCs were assessed by flow cytometry and reported in the histogram. Samples were run after 72 h of incubation with or without BM-MSCs. (C) The CD4/CD8 ratio was calculated from data obtained by flow staining in samples of healthy activated PBMCs alone and after coculture with BM-MSCs. (D) Detection of CD4+CD25highFoxp3+ fraction in purified T cells collected from healthy volunteers and stimulated with anti-CD3/CD28 for 3 days with or without BM-MSCs. In all experiments unstimulated PBMCs were used as negative controls. Data are presented as means ± SE in each histogram; *p