6533b838fe1ef96bd12a3ba2

RESEARCH PRODUCT

Ex vivo high resolution magic angle spinning metabolic profiles describe intratumoral histopathological tissue properties in adult human gliomas.

M.c Martinez-bisbalBernardo CeldaS. Van HuffelJ. M. CerdaA. Croitor SavaDiana M. Sima

subject

AdultPathologymedicine.medical_specialtyMagnetic Resonance SpectroscopyNecrosisBrain NeoplasmsReference tissueChemistryBiopsyMetaboliteHigh resolutionGliomaIn Vitro TechniquesNecrotic tissueMalignancy gradechemistry.chemical_compoundMagic angle spinningmedicineHumansRadiology Nuclear Medicine and imagingmedicine.symptomEx vivo

description

In gliomas one can observe distinct histopathological tissue properties, such as viable tumor cells, necrotic tissue or regions where the tumor infiltrates normal brain. A first screening between the different intratumoral histopathological tissue properties would greatly assist in correctly diagnosing and prognosing gliomas. The potential of ex vivo high resolution magic angle spinning spectroscopy in characterizing these properties is analyzed and the biochemical differences between necrosis, high cellularity and border tumor regions in adult human gliomas are investigated. Statistical studies applied on sets of metabolite concentrations and metabolite ratios extracted from 52 high resolution magic angle spinning recordings coming from patients with different grades of glial tumors show a strong correlation between the histopathological tissue properties and the considered metabolic profiles, regardless of the malignancy grade. The results are in agreement with the pathology obtained by the histopathological examination that succeeded the high resolution magic angle spinning measurements. The metabolite concentration set can better differentiate between the considered histopathological tissue properties compared to the ratios. Representative reference tissue models describing the metabolic behavior are extracted for characterizing the intratumoral tissue properties. The proposed metabolic profiles reflect that the metabolites behavior is interconnected, and typical biochemical patterns emerge for each histopathological tissue property. Magn Reson Med, 2011. © 2010 Wiley-Liss, Inc.

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