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RESEARCH PRODUCT

Preventive effects of n-3 polyunsaturated fatty acids in alteration of colon mucus layer and reticulum endoplasmic stress induced by an obesogenic diet

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Obesity is now recognized as a real global epidemic affecting more than 650 million people worldwide.This chronic inflammatory disease is accompanied with intestinal alteration, including alteration of the colic mucus layer and an increase in stress of the endoplasmic colic reticulum, associated with microbiota dysbiosis. Polyunsaturated fatty acids in n-3 are known for their anti-inflammatory and immuno-modulatory properties and appear to be a promising lead in the fight against metabolic and intestinal alterations induced by obesity.The objectives of this work was initially to determine whether tissue enrichment in n-3, in the fat-1 mouse model, prevent alterations of colon mucus layer induced by an obesogenic diet and understood the molecular mechanisms involved. We then sought to determine, through caecal microbiota transplantation, whether fat-1 mouse microbiota was “independently involved” in preventing obesity-induced alterations in fat-1 mice.In conclusion, this work shows that polyunsaturated fatty acids in n-3 prevent colon mucus layer alteration by preventing increased of endoplasmic reticulum stress induced by an obesogenic diet. We also showed that the intestinal microbiota of fat-1 mice, modulated by omega-3 fatty acids, actively participated in the prevention of alterations induced by obeesogenic conditions. These results present the modulation of the intestinal microbiota by omega-3 fatty acids as a promising way in the prevention of metabolic and intestinal alterations induced by an obesogenous diet.