6533b838fe1ef96bd12a46ee
RESEARCH PRODUCT
Fluxapyroxad Haptens and Antibodies for Highly Sensitive Immunoanalysis of Food Samples
Josep V. MercaderConsuelo AgullóAntonio Abad-fuentesAntonio Abad-somovillasubject
Calibration curvemedicine.drug_classFungicideCarboxamideEnzyme-Linked Immunosorbent AssayFood ContaminationFluxapyroxad01 natural sciencesSensitivity and SpecificityAntibodiesFood safetymedicineChemical residuesDetection limitPrunus persicaChromatographymedicine.diagnostic_test010405 organic chemistryChemistrybusiness.industry010401 analytical chemistryGeneral ChemistryFood safetyAmides0104 chemical sciencesFungicides IndustrialFruit and Vegetable JuicesImmunoassayFruitMalusELISAGeneral Agricultural and Biological SciencesFood qualitybusinessHaptenHaptensdescription
Fluxapyroxad is a new-generation carboxamide fungicide, with residues increasingly being found in food samples. Immunochemical assays have gained acceptance in food quality control as rapid, cost-effective, sensitive, and selective methods for large sample throughput and in situ applications. In the present study, immunoreagents to fluxapyroxad were obtained for the first time, and competitive immunoassays were developed for the sensitive and specific determination of fluxapyroxad residues in food samples. Two carboxyl-functionalized analogues of fluxapyroxad were prepared, and antibodies with IC50 values in the low nanomolar range were generated from both haptens, though a dissimilar response was observed concerning specificity. A robust direct assay was set up, with a calibration curve exhibiting a limit of detection of 0.05 nM (0.02 μg/L). Limits of quantitation of 5 μg/L were obtained for peach, apple, and grape juices using samples diluted in water. The direct immunoassay was also successfully applied to the determination of fluxapyroxad in grapes from in-field treated grapevines.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-10-12 |