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RESEARCH PRODUCT

Seed cryopreservation of Fraxinus angustifolia Vahl

Anna ScialabbaRosario SchicchiGiovanna Lombardo

subject

GermplasmFraxinus angustifoliaBiologybiology.organism_classificationApplied Microbiology and BiotechnologyCryopreservationFraxinus angustifolia germination germplasm conservation seed cryopreservationSettore BIO/01 - Botanica Generalechemistry.chemical_compoundAgronomychemistryGerminationSettore BIO/03 - Botanica Ambientale E ApplicataGeneticsDormancyAgronomy and Crop ScienceMolecular BiologyGibberellic acidWater contentBiotechnology

description

Fraxinus angustifolia is used for afforestation and for production of both valuable timber and manna , a substance with pharmacological applications. The aim of this research was to establish the optimized condition for cryopreservation of F. angustifolia seeds, in order to protect the germplasm of this species. Germination percentage and mean time of germination of non-treated seeds (control) and liquid nitrogen-treated seeds were established in water or in 10 -6 M gibberellic acid (GA 3 ). The seeds could be cryopreserved with 3% of moisture content (MC) and germinated easily in water (70.0 ± 5.0%), while seeds cryopreserved with 6% MC showed a physiological dormancy. This state could be efficiently removed by addition of GA 3 , which increased germination to 76.0 ± 2.4%. Liquid nitrogen did not impair embryo viability, allowing a successful cryopreservation of seeds. The results are particularly useful for both germplasm conservation programs and in narrow leaved ash nurseries. Keywords : Fraxinus angustifolia , germination, germplasm conservation, seed cryopreservation African Journal of Biotechnology Vol. 12(16), pp. 1930-1936

yearjournalcountryeditionlanguage
2016-01-28
10.5897/ajb12.2866http://hdl.handle.net/10447/73376