6533b838fe1ef96bd12a47f9

RESEARCH PRODUCT

Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus

Fabio CaradonnaAnn Maria Clementea.m. ClementeDaniele BellaviaGiorgia SisinoRainer Barbieri

subject

DNA RibosomalChromosomesParacentrotus lividusGene mappingbiology.animalGeneticsmedicineAnimals5S rDNA Paracentrotus lividusCloning MolecularMolecular BiologySea urchinRibosomal DNAIn Situ Hybridization FluorescenceSouthern blotGeneticsbiologymedicine.diagnostic_testRNA Ribosomal 5SChromosome MappingGeneral MedicineRibosomal RNAbiology.organism_classificationMolecular biologySettore BIO/18 - GeneticaParacentrotusPloidyBiotechnologyFluorescence in situ hybridization

description

In this paper the chromosomal localization and molecular cloning and characterization of three 5S rDNA clusters of 700 bp (base pairs), 900 bp, and 950 bp in the sea urchin Paracentrotus lividus are reported. Southern blot hybridization demonstrated the existence of three 5S rDNA repeats of differing length in the P. lividus genome. Fluorescence in situ hybridization analysis, performed in parallel on both haploid and diploid metaphases and interphase nuclei using different 5S rDNA units as probes, localized these 5S rDNA clusters in 3 different pairs of P. lividus chromosomes. This is the first complete gene mapping not only in a sea urchin but also in the phylum of echinoderms as a whole.

yearjournalcountryeditionlanguage
2007-09-26Genome
https://doi.org/10.1139/g07-062