6533b838fe1ef96bd12a48a9
RESEARCH PRODUCT
TheGCA1gene encodes a glycosidase-like protein in the cell wall ofCandida albicans
Rafael SentandreuEulogio ValentínAntonio CamineroJosé P. MartínezSergi Maicassubject
0301 basic medicineGlycosylationGlycoside HydrolasesGenes Fungal030106 microbiologyMutantCalcofluor-whiteApplied Microbiology and BiotechnologyMicrobiologyCell wallGene Knockout Techniques03 medical and health scienceschemistry.chemical_compoundGlucosidesCell WallCandida albicansCandida albicanschemistry.chemical_classificationMicrobial ViabilitybiologyGeneral Medicinebiology.organism_classificationMolecular biologyEnzyme assayCorpus albicansEnzymechemistryBiochemistrybiology.proteindescription
Candida albicans Gca1p is a putative glucoamylase enzyme which contains 946 amino acids, 11 putative sites for N -glycosylation and 9 for O -glycosylation. Gca1p was identified in β-mercaptoethanol extracts from isolated cell walls of strain C. albicans SC5314 and it is involved in carbohydrate metabolism. The significance and the role of this protein within the cell wall structure were studied in the corresponding mutants. The homozygous mutant showed that GCA1 was not an essential gene for cell viability. Subsequent phenotypic analysis performed in the mutants obtained did not show significant difference in the behavior of mutant when compared with the wild strain SC5314. Zymoliase, Calcofluor White, Congo red, SDS, caffeine or inorganic compounds did not affect the integrity of the cell wall. No differences were observed when hyphal formation assays were carried out. However, an enzyme assay in the presence of substrate p -nitrophenyl-α-D-glucopyranoside enabled us to detect a significant decrease in glycosidase activity in the mutants compared with the parental strain, revealing the function of Gca1.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-04-05 | FEMS Yeast Research |