6533b838fe1ef96bd12a48d4
RESEARCH PRODUCT
Ion-exchange high-performance liquid chromatography of nucleotides and polypeptides on new types of ion-exchange sorbents, based on polystyrene-coated silicas.
Vadim A. DavankovA. A. KurganovK.k. Ungersubject
Inorganic chemistryCovalent bindingBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryIonchemistry.chemical_compoundAnimalsHumansNucleotidePorosityChromatography High Pressure Liquidchemistry.chemical_classificationChromatographyIon exchangeNucleotidesOrganic ChemistryGeneral MedicineChromatography Ion ExchangeSilicon DioxidechemistryPolystyrenesCattlePolystyreneIon Exchange ResinsPeptidesLayer (electronics)description
A novel type of ion exchanger was prepared by multipoint covalent binding of polystyrene chains onto the surface of porous silica followed by polymer-analogous modification of the bonded layer. Both anion and cation exchangers were synthesized and examined in the separation of nucleotides and proteins. Rapid and efficient separation of basic polypeptides on strong anion exchangers and that of acidic polypeptides on strong cation exchangers could be achieved. For the separation of complete mixtures of polypeptides the application of zwitter-ionic ion exchangers can be recommended.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-07-01 | Journal of chromatography |