6533b838fe1ef96bd12a5230

RESEARCH PRODUCT

Microstructuring of phospholipid bilayers on gold surfaces by micromolding in capillaries

Steffen SchuyAndreas Janshoff

subject

Materials sciencePolydimethylsiloxaneLipid BilayersMicrofluidicsMicrofluidicsSiliconestechnology industry and agriculturePDMS stampNanotechnologyMicroscopy Atomic ForceSoft lithographySurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsBiomaterialschemistry.chemical_compoundColloid and Surface ChemistrychemistryMonolayerDimethylpolysiloxanesGoldSelf-assemblyLipid bilayerPOPCPhospholipids

description

Microstructuring of lipid bilayers on gold surfaces was achieved by micromolding in capillaries employing chemically modified polydimethylsiloxane (PDMS). Microfluidic networks of PDMS were prepared by micromolding and functionalized with thiol end-groups using 3-mercaptopropyltrimethoxysilane. The PDMS stamps were firmly attached to the gold substrate via quasi-covalent linkage providing a tight seal, a prerequisite for establishing individual addressable capillaries. Bilayers composed of POPC/POPG were subsequently prepared on microstructured self assembly monolayers of 11-amino-1-undecanethiol via strong electrostatic interactions. This way it is possible to generate individually addressable lipid bilayers on gold surfaces, a procedure, which is of widespread interest for investigating protein lipid interactions with microscopic techniques.

yearjournalcountryeditionlanguage
2005-06-10Journal of Colloid and Interface Science
https://doi.org/10.1016/j.jcis.2005.07.071