6533b838fe1ef96bd12a5268
RESEARCH PRODUCT
Clostridium difficile IStron CdISt1: Discovery of a Variant Encoding Two Complete Transposase-Like Proteins
Claudia NitscheChristoph Von Eichel-streiberMichael MoosVeit BraunMaja RupnikOliver Hasselmayersubject
Genetics0303 health sciencesbiology030306 microbiologyClostridioides difficileStrain (biology)Bacteriophages Transposons and PlasmidsMolecular Sequence DataRibozymeIntronTransposasesClostridium difficilebiology.organism_classificationMicrobiologyIntrons03 medical and health sciencesGenes Bacterialbiology.proteinBacteriologyDNA Transposable ElementsClostridiaceaeInsertion sequenceMolecular BiologyTransposase030304 developmental biologydescription
ABSTRACT Screening a Clostridium difficile strain collection for the chimeric element Cd ISt1 , we identified two additional variants, designated Cd ISt1 -0 and Cd ISt1 -III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known Cd ISt1 variants led us to define four types of IStrons that we designated Cd ISt1 -0 through Cd ISt1 -III. Since Cd ISt1 -0 encodes two complete transposase-like proteins (TlpA and TlpB), we suggest that it represents the original genetic element, hypothesized before to have originated by fusion of a group I intron and an insertion sequence element.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-04-15 |