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RESEARCH PRODUCT
Thioflavin T Hydroxylation at Basic pH and Its Effect on Amyloid Fibril Detection
Marco Van De WeertMinna GroenningFabio LibrizziVito FoderàLars Folke OlsenSalvatore SpagnoloValeria VetriClaus CornettMaurizio Leonesubject
AmyloidMagnetic Resonance SpectroscopyAqueous solutionTemperatureThioflavin T AmyloidHydrogen-Ion ConcentrationHydroxylationPhotochemistryFibrilFluorescenceFluorescence spectroscopySurfaces Coatings and FilmsHydroxylationKineticsThiazoleschemistry.chemical_compoundchemistryMaterials ChemistryProton NMROrganic chemistrySpectrophotometry UltravioletThioflavinChemical stabilityBenzothiazolesPhysical and Theoretical Chemistrydescription
The fluorescent dye thioflavin T (ThT) is commonly used for in situ amyloid fibril detection. In this work, we focused on the spectroscopic properties and chemical stability of ThT in aqueous solution as a function of pH, temperature, and dye concentration. A reversible hydroxylation process occurs in alkaline solutions, which was characterized using a combination of UV-vis absorption spectroscopy, proton NMR, and density functional theory (DFT). On the basis of these studies, we propose a chemical structure for the hydroxylated form. Finally, by means of fluorescence spectroscopy, ThT hydroxylation effects on in situ amyloid detection have been investigated, providing new insights on the efficiency of the ThT assay for quantitative fibril evaluation at basic pH.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-10-30 | The Journal of Physical Chemistry B |