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RESEARCH PRODUCT

FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

Miriam GaggianesiLe ZhangJan Paul MedemaSimone Di FrancoGiorgio StassiMelania Lo Iacono

subject

Science (General)Colorectal cancerTumor initiationBiologyGeneral Biochemistry Genetics and Molecular BiologyQ1-390Cancer stem cellmedicineProtocolHumansFlow Cytometry/Mass CytometryClonogenic assayWnt Signaling PathwayCancerGeneral Immunology and MicrobiologyGeneral NeuroscienceStem CellsWnt signaling pathwayCancerCell Biologymedicine.diseaseFlow CytometryMolecular/Chemical ProbesCancer cellcolorectal cancers cancer stem cells FACS Wnt.Cell isolationCancer researchNeoplastic Stem CellsCell-based AssaysStem cellSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioColorectal NeoplasmsSignal Transduction

description

Summary Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).

yearjournalcountryeditionlanguage
2021-12-01STAR Protocols
10.1016/j.xpro.2021.100880http://www.sciencedirect.com/science/article/pii/S2666166721005864