6533b839fe1ef96bd12a6395

RESEARCH PRODUCT

Polymorphisms in the intergenic region of the sea urchin Paracentrotus lividus ribosomal DNA

Maria CantoneSilvana VenturellaGiovanni DuroRainer BarbieriVincenzo IzzoGiovanni Giudice

subject

Geneticseducation.field_of_studybiologyPopulationCell Biologybiology.organism_classificationParacentrotus lividusgenomic DNARestriction siteRestriction mapIntergenic regionbiology.animaleducationRibosomal DNASea urchin

description

Abstract Blot-hybridizations of the sea urchin Paracentrotus lividus genomic DNA with ribosomal DNA (rDNA) probes revealed individual variations in the length and in the sequence of the non-transcribed spacer (NTS) region. The number of rDNA repeat subclasses distinguishable within any individual sea urchin is usually limited (1 to 3) with respect to the widest polymorphism of the population as a whole. The heterogeneity in sequence is revealed by the presence or the absence of specific restriction sites in the spacer region. The data obtained by the intensity of the polymorphic bands indicate that different mechanisms bring about these two types of polymorphism. Preliminary data also indicate different methylation patterns in single individuals.

yearjournalcountryeditionlanguage
1990-10-01Cell Biology International Reports
https://doi.org/10.1016/0309-1651(90)91161-v