6533b839fe1ef96bd12a6683
RESEARCH PRODUCT
Liposomes as nonspecific nanocarriers for 5-Fluorouracil in the presence of cyclodextrins
Aneta BuczekDanuta PentakMałgorzata A. BrodaTeobald KupkaSławomir Maślankasubject
Release mechanismLiposomeCyclodextrins5-Fluorouraciltechnology industry and agricultureCondensed Matter PhysicsCombinatorial chemistryAtomic and Molecular Physics and OpticsFluorescence spectroscopyElectronic Optical and Magnetic MaterialsNMR spectra databasechemistry.chemical_compoundDifferential scanning calorimetrychemistryDipalmitoylphosphatidylcholineLiposomesMaterials ChemistryProton NMRMoleculelipids (amino acids peptides and proteins)Physical and Theoretical ChemistryNanocarriersSpectroscopyControlled drug deliverydescription
Abstract 5-Fluorouracil (5-FU) is one of anticancer drugs with broad activity. Due to its severe side effects, recent studies concentrate on new ways of directed 5-FU delivery and its release in ill tissue. One of selective carriers could be cyclodextrins and liposomes. The combination of novel methods, leading to formation of inclusion complexes (IC) between host molecule of β-cyclodextrin (β-CD) or 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and 5-FU guest and its subsequent encapsulation in dipalmitoylphosphatidylcholine (DPPC) liposomes is studied experimentally in the present work. Several methods are applied to proof the encapsulation of the analysed drug and its release over time at 37 and 41 °C. The 5-FU release from complex of liposomal form of 5-fluorouracil and β-cyclodextrin (L[DPPC/5-FU]:β-CD) and complex of liposomal form of 5-fluorouracil and (2-hydroxypropyl)-β-cyclodextrin (L[DPPC/5-FU]:HP-β-CD) was followed using UV–Vis and fluorescence spectroscopy, proton nuclear magnetic resonance (1H NMR) and differential scanning calorimetry (DSC) techniques. The electronic and NMR spectra analysis were additionally supported by molecular modeling, including prediction of spectral changes in vacuum and in water using density functional theory (DFT-B3LYP-D3). From the observed changes the fastest release from liposomes (within 1 h) was observed for the 5-FU complexes with HP-β-cyclodextrin at 41 °C.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-12-01 | Journal of Molecular Liquids |