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RESEARCH PRODUCT
False-negative results by polymerase chain reaction due to contamination by glove powder
J G De LomasM.p. BuschM.p. BuschF J SunzeriF J Sunzerisubject
MalePathologymedicine.medical_specialtyDrug ContaminationChemistryImmunologyFalse Negative Reactionsnutritional and metabolic diseasesHematologyContaminationPolymerase Chain ReactionMicrobiologylaw.inventionlawmedicineNucleic acidHumansImmunology and AllergyGloves SurgicalPowdersDrug ContaminationFalse Negative ReactionsPolymerase chain reactiondescription
The polymerase chain reaction (PCR) technique has become an important, widely employed method for the detection and quantitation of the nucleic acid sequences used in the diagnosis and monitoring of genetic and infectious diseases. Much attention has been directed at the problem of false-positive PCR results, which are generally attributed to low-level laboratory contamination of amplified sequences ("carryover"). In contrast, few investigators have commented on the somewhat less frequent, but equally problematic, false-negative PCR results. Investigation of the source of sporadic false-negative PCR reactions found that glove powder, inadvertently introduced into tubes when gloves are changed in an effort to reduce false-positive results, can nonspecifically inhibit each of the major steps in the PCR detection process. Methodologic precautions are recommended to minimize this problem.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-01-01 | Transfusion |