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RESEARCH PRODUCT

Detection of Human Papillomavirus DNA in Cervical Samples: Analysis of the New PGMY-PCR Compared To the Hybrid Capture II and MY-PCR Assays and a Two-Step Nested PCR Assay

Pietro AmmatunaLucia GiovannelliAnna LamaGiuseppina CapraPietro AricoViviana Giordano

subject

Microbiology (medical)GenotypeTwo stepPcr assayCervix UteriBiologyPolymerase Chain ReactionSensitivity and Specificitylaw.inventionlawVirologyHuman papillomavirus DNAHumansHuman papillomavirusPapillomaviridaePolymerase chain reactionPapillomavirus InfectionsHybrid capturevirus diseasesVirologyMolecular biologyfemale genital diseases and pregnancy complicationsTumor Virus InfectionsHpv testingDNA ViralFemaleNested polymerase chain reaction

description

ABSTRACT The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR ( n PCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative- n PCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.

https://doi.org/10.1128/jcm.42.8.3861-3864.2004