6533b83afe1ef96bd12a70f1
RESEARCH PRODUCT
Detection of Human Papillomavirus DNA in Cervical Samples: Analysis of the New PGMY-PCR Compared To the Hybrid Capture II and MY-PCR Assays and a Two-Step Nested PCR Assay
Pietro AmmatunaLucia GiovannelliAnna LamaGiuseppina CapraPietro AricoViviana Giordanosubject
Microbiology (medical)GenotypeTwo stepPcr assayCervix UteriBiologyPolymerase Chain ReactionSensitivity and Specificitylaw.inventionlawVirologyHuman papillomavirus DNAHumansHuman papillomavirusPapillomaviridaePolymerase chain reactionPapillomavirus InfectionsHybrid capturevirus diseasesVirologyMolecular biologyfemale genital diseases and pregnancy complicationsTumor Virus InfectionsHpv testingDNA ViralFemaleNested polymerase chain reactiondescription
ABSTRACT The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR ( n PCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative- n PCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.
year | journal | country | edition | language |
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2004-08-07 | Journal of Clinical Microbiology |