0000000000852280

AUTHOR

Viviana Giordano

showing 2 related works from this author

Phylogenetic reconstruction of HCV genotype 1b dissemination in a small city centre: The Camporeale model

2008

Several seroepidemiological population-based surveys carried out in Italy have shown a high prevalence of hepatitis C virus (HCV) infection. Camporeale (CP), a small Sicilian town with a 10.4% prevalence of HCV mostly genotype 1b, probably represents a specific context, since intravenous drug addiction, and sexual promiscuity are almost absent. In order to reconstruct the pattern of introduction and diffusion of HCV in this ecological niche, the NS5 genomic region of 72 HCV genotype 1 isolates (39 from CP and 33 collected throughout Sicily) was amplified and sequenced. Sequences were aligned and analyzed by BioEdit, PAUP and BEAST, and their molecular evolution compared. Thirty-eight HCV ge…

MaleSettore MED/07 - Microbiologia E Microbiologia ClinicaUrban PopulationSequence analysisIatrogenic DiseasePopulationHepacivirusViral Nonstructural Proteinsmolecular epidemiologyMonophylyFlaviviridaecoalescent inference analysiPhylogeneticsVirologyGenotypePrevalenceCluster AnalysisHumanshepatitis C virueducationSicilyPhylogenyAgedGeneticsSettore MED/12 - Gastroenterologiaeducation.field_of_studyMolecular epidemiologybiologyPhylogenetic treeSequence Analysis RNAiatrogenic routeBayes TheoremHepatitis C ChronicMiddle Agedbiology.organism_classificationHepatitis CVirologyInfectious DiseasescommunityFemaleMonte Carlo MethodJournal of Medical Virology
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Detection of Human Papillomavirus DNA in Cervical Samples: Analysis of the New PGMY-PCR Compared To the Hybrid Capture II and MY-PCR Assays and a Two…

2004

ABSTRACT The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR ( n PCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative- n PCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.

Microbiology (medical)GenotypeTwo stepPcr assayCervix UteriBiologyPolymerase Chain ReactionSensitivity and Specificitylaw.inventionlawVirologyHuman papillomavirus DNAHumansHuman papillomavirusPapillomaviridaePolymerase chain reactionPapillomavirus InfectionsHybrid capturevirus diseasesVirologyMolecular biologyfemale genital diseases and pregnancy complicationsTumor Virus InfectionsHpv testingDNA ViralFemaleNested polymerase chain reactionJournal of Clinical Microbiology
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