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RESEARCH PRODUCT
Analysis of Diuretics in Urine by Column-Switching Chromatography and Fluorescence Detection
Rosa Herráez-hernándezMaria Dolores Pastor-navarroPilar Campí Ns-falcósubject
TriamtereneChromatographyChemistrymedicine.drug_classmedicine.medical_treatmentClinical BiochemistryPharmaceutical ScienceUrineBiochemistryHigh-performance liquid chromatographyFluorescence spectroscopyAnalytical ChemistryPotassium-sparing diureticmedicineDiureticQuantitative analysis (chemistry)Bumetanidemedicine.drugdescription
The potential of column-switching chromatography and fluorescence detection for the analysis of diuretics in urine is evaluated. Sample cleanup and chromatographic parameters have been optimized to achieve maximum sensitivity for the detection and quantification of some relevant diuretics. On the basis of these studies, an on-line procedure for the simultaneous determination of amiloride, furosemide, bumetanide and triamterene is presented. The linearity, precision, accuracy and sensitivity of the method are discussed. The utility of the described approach has been tested by analysing urine samples obtained after administration of bumetanide.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-07-01 | Journal of Liquid Chromatography & Related Technologies |